Training dogs to distinguish between black bear (Ursus americanus) and grizzly bear (Ursus arctos) feces

 

 

Aimee Hurt

The University of Montana IBS-CORE Program

Mentors- Barbara Davenport and Erick Greene

 

 

 

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Executive Summary

The last decade has seen an increase in the use of dogs by wildlife researchers and managers. In addition to the commonplace use of dogs by police officers and search and rescue crews, dogs have been used to monitor wolf packs, manage human-habituated bears, and locate the feces (scat) of endangered species so that it could be analyzed for genetic information.

 

Grizzly bears are an endangered species and the focus of many research projects. However, their scat is not visually distinguishable from the scat of black bears, a relatively abundant species that is not endangered. Dogs who could distinguish between the two would be valuable resources as a pre-lab litmus test of species identification. Our study successfully trained three dogs to distinguish between grizzly bear and black bear scat when presented together in a scent box—a common apparatus used for narcotic detection training. While the overall success rates of 65% for one dog and 85% for the other two were fairly high, they were even higher in rounds that contained familiar grizzly scat. Because of this we concluded that dogs must be trained with a much greater diversity of grizzly scat to increase their ability to generalize grizzly scat odor. Additionally they need more hours of training than we provided them. Finally, to withstand the repetitive rigor of this type of detection work, the dogs must be carefully selected for this work.

 

Introduction

Nearly one-eighth of a dog’s brain is dedicated to olfaction. A dog’s nose contains up to 220 million olfactory sensory cells, whereas man has about 5 million (Syrotuck 1972). Consequently, trained scent detection dogs are utilized in many professional applications. Trained dogs are used to locate narcotics, lost or buried people, and explosives. Additionally dogs have been used to locate termites, spoiled wine, truffles, cancerous skin areas, and wildlife feces (scat).

 

In the last decade scientists have explored the limits and possibilities of canine olfaction (Brisbin and Austad 1991), and the use of dogs as tools for wildlife researchers and managers has grown. Dogs have been trained to locate wolf sign (tracks, scat, urine) for the monitoring of pack locations and interactions (Sturdvian 1993); as well as the highly publicized use of dogs for the aversive conditioning of habituated bears (Hunt 1997). Dogs have also been used to find black bear, grizzly bear, and wolf scat (Wasser and Davenport unpublished) and kit fox scat (Smith in progress) in order to analyze collected scat for genetic information. 

 

We aimed to advance the use of dogs in wildlife research by training them to distinguish between the scat of black bears and grizzly bears. Currently, scat samples are identified for species only in the laboratory, as such a differentiation cannot be made by visual inspection. If dogs show the capacity for this level of distinction then they can be used as an inexpensive litmus test to assist wildlife biologists and managers in their efforts to monitor wildlife populations.

 

 

Methods

Experimental Subjects

Dogs were selected for their ball driven (object-oriented) nature.  Dogs who were living as household pets were used as well as kenneled dogs who were candidates for narcotic detection dog training. Nine dogs were selected for training, 6 pets and 3 kenneled narcotic candidates, ranging in scent experience from none to 5 weeks of a 6-week narcotic detection training course. Ages ranged from 1year to approximately 5 years. One dog was a yellow Labrador Retriever (Kelly), one an Australian Shepherd (Zach), two Golden Retrievers, and all others were mixed breeds (Maverick). Training and testing was conducted in August through October 2000, at two separate indoor locations. 

 

Training Method

The training method was based on the scent box, a widely used training apparatus for the training of narcotic detection dogs. At the first location, the scent box was a box of untreated ¾”plywood (43” long, 10” wide, 6.5” deep), separated into 5 compartments by a single hinged lid. When the lid was closed the holes (3” holes spaced 9” apart center to center) allowed access to each compartment. At the second location, the scent box was made of ¾” melamine and was 4 individual boxes (8” long, 7” wide, 7” deep) that could latch together to act as a single box. Each box had a 2 ½” hole in the lid. The dog was trained to display a passive (“Sit”) response at recognition of the grizzly scat (target odor) by waiting for the ball reward and not pawing the sample.

 

Initially one grizzly sample—contained in a plastic vial with an open lid—was placed in one of the holes of the scent box. The other holes contained only empty plastic vials, identical to the vial that contained the scat. The dog was led on leash to the correct hole and encouraged by hand presentation and voice command to sniff that hole. Immediately the ball was thrown across the dog’s line of vision and the dog was allowed to retrieve the ball and was played with and verbally praised for a minute or so. This step acquainted the dog with the scent box and began the process of associating grizzly scat odor with the ball.

 

Next the dog was encouraged to check other holes before being led to the correct hole and rewarded. This allowed the dog to understand the concept of searching the entire box. Additionally he learned to associate the odor with the ball reward, rather than with the act of smelling a hole. The correct hole was then moved to other locations within the dog’s search, in order to discourage the expectation that the target scat would be in a specific location.

 

The use of the scent box allowed the dog to develop a good pinpoint (nose in or directly over hole) as it allowed for the use of a large quantity of scat. Additionally, the hole was an obvious visual cue for the dog. However, further training required the ability to move small amounts of scat in and out quickly. The scent box was not amenable to this due to its tendency to retain odor (thus contaminating subsequent trials by lingering odor). Therefore, at this stage B.D changed the training apparatus to half-pint canning jars set within the holes of the scent-box so that the lip remained above the surface of the box. Jars were fitted with cardboard “washers” to attain a proper fit in the hole. Two 5-holed scent boxes were placed end to end and a jar was placed every other hole, so that a total of 5 jars were each placed 18” apart. Empty holes were covered with cardboard. At the second training location the jar apparatus was created using two 2x4 pieces of lumber nailed together with wooden spacers to allow the jars to slide between them and remain with the lip above the boards. Again the jars were set at 18” apart and the entire space between them was covered with cardboard.

 

The dog was led across the jars—one of which contained approximately 50mL of grizzly scat—until it was clear that the change in apparatus was not a hindrance to the dog. When the dog displayed a satisfactory recognition of several grizzly scats we began conflict training. Generally, conflict training is the introduction of odors for which dog is not rewarded. For this training the conflict odor was black bear scat. One jar was filled with approximately 25mL of black bear scat—a lesser amount than the grizzly so that the grizzly odor was the overwhelming odor. The black bear jar was located 2 jars away from the grizzly jar. The dog was led on leash to the box so that he would encounter the black first; if he continued to smell the black bear or give a positive response he was given a correction (snap of the collar) and told “no”. The dog was then led along the box and when he smelled the grizzly he was rewarded with the ball and abundant verbal praise. This was repeated moving the scats (target and conflict) around between each approach. The amount of black bear in each jar was increased to equal the grizzly, about 50mL. Various scats were presented and complexity increased until all 5 holes were filled with scat, 1 grizzly and 4 black bear.

 

When the dog showed proficiency at hitting on the target scat the assistant then moved the scat around and rounds were completed without the handler knowing which jar contained the target scat. This removed the possibility that the handler was cueing the dog.  At this advanced stage of training, the dog was occasionally given rounds in which there was no grizzly scat present. When the dog accurately found nothing he was rewarded with strong verbal praise but no ball.  Different grizzly scats were used throughout training to encourage the dog to generalize grizzly odor and recognize it in all forms. The assistant contaminated the jars with human odor when picking them up to switch them in and out, and was therefore careful to handle all jars equally so that the dog did not associate human odor with a reward.  Each dog was trained for no more than 20 minutes at a time before taking a break, and more often for less than 15 minutes.

 

At all stages the dog was encouraged to sit upon smelling the correct hole so that he presented a clear sign to the handler that he had located the scat. He was encouraged to re-smell the correct hole with a verbal “show me” command. A dog who did not know the sit command was not forced to sit, as it was inappropriate from a training standpoint to insert obedience lessons into the middle of a scent training session which must remain fun and brief.  When lacking a sit, the dog naturally developed another signal (looking at handler, staring into hole with ear-set moved forward) that the handler learned to read.

 

The use of leash, the proximity of handler to dog, and the extent of hand presentations did not remain constant throughout training. Instead these were dictated primarily by the working style of the dog, through which preferences were displayed by improved concentration, confidence, or interest in working. 

 

 

Experimental Protocol

At the first location B.D handled both Kelly and Maverick during training and testing. A.H handled Zach at the second location. For testing, 25 rounds were randomly configured with 4 jars containing black bear and one containing grizzly for 20 rounds, and 5 jars holding black bear and no grizzly for 5 rounds. These “blank” rounds were nearly randomly distributed, selected only so that they did not occur consecutively. From a training aspect, the blank rounds were potentially demoralizing for the dog because he was essentially working for no reward. An experienced detection dog could withstand this, but with novice dogs care was taken to temper the impact of no reward. The assistant placed out the scat according to the random assignment, so the handler was unaware of the location of the target scat. The handler led the dog to the jar-box starting at the first jar and working down to the fifth jar. The handler had full discretion in how many times to lead the dog back and forth along the box before deciding that the round was complete. The dog indicated on a hole and the handler verified with the assistant that the hole was correct before rewarding the dog, to avoid rewarding in error. If the dog was incorrect then the assistant said no, and the handler and dog continued down the box. The false response was recorded by the assistant for that round as well as the subsequent “hit” or “miss” depending on whether or not the dog ended up choosing the correct scat. If the handler declared the round complete and the target scat had been missed the assistant told the handler the location of the target scat so that the dog could be brought to it and rewarded. The dog was given a several minute break in between rounds.  Each dog encountered the same 25 configurations in a different order.

 

All training and testing was videotaped. All scats were frozen as soon after collection as possible. They were transferred from collection bags into vials using latex gloves.

 

Results

During the testing, dogs were exposed to a total of 33 scats (see table 1), 13 grizzly and 20 black bear. 16 (6 grizzly and 10 black bear) were the scats that were used during training. The 13 grizzly scats came from 4 different animals, all captive. Dogs were trained with 6 scats from 2 individuals (from same facilities with same diets) and were then tested using the training scats as well as 7 other scats—3 from the individuals they were trained on and 4 scats from 2 individuals they had not encountered in training. The 20 back bear scats were both wild and captive. Dogs were trained on 10 black bear scats from 7 individuals (two black bears had the same diet as the two grizzlies used for training). Testing included these scats as well as 3 additional captive scats from the animals they had been trained on and 7 new wild black bear scat that they had not previously encountered.

 

Hit rates (see table 2) were broken down into categories depending upon the scat used: scat that was used to train the dogs (Known); scat that was from individuals that the dogs had never encountered (Unknown); new scat from known individuals (New from known bears); and multiple scat from bears which were unknown to dogs prior to testing day, but after dogs encountered them in training rounds became potentially “known” to a limited extent (New on testing day). A single grizzly scat appeared from 1-3 times throughout testing.

 

The false response rate (hitting on black bear) was categorized as follows: the number of rounds the dogs gave a false response when there was grizzly to be found in that round (FR when griz available); the number of rounds the dog gave a false response when there was no grizzly to be found (FR when no griz available); the number of individual false responses on black bear scat the dog had encountered in training (FR on known black bear); and the number of individual false responses on black bear scat new to the dog that day (FR on new black bear).

 

It should be noted that this form of testing did not allow for complete independence of rounds due to the ability of the dog to learn from one round and potentially succeed accordingly on subsequent encounters with that scat. A dog who missed a sample in one round would learn, by lack of reward, that he was correct to pass it up (negative learning). A dog who was led back to a sample after missing it and then rewarded, potentially learned to correctly hit that scat in subsequent encounters (positive learning). Because the potential for learning existed either way we chose positive learning. Given this, note that the statistical analysis is not a perfect examination of the testing. That said, results indicate that dogs selected grizzly bear at a rate far exceeding random (for p=.85, and p=.65 both significant in the extreme case of a=.001). Overall hit rates for Maverick equaled 65% and 85% for Zach and Kelly. When broken down, the most notable hit rates of 89-100% occurred when dogs hit on known scat. The hit rates for new on training day scat ranged from 50-100%. There were only two unknown scats, Maverick missed both of them, while Kelly and Zach correctly identified one of the two. Of the three scats from known bears, Maverick selected one, Kelly two, and Zach all three. The number of rounds in which the dogs gave at least one false response were dramatically different in rounds where there was grizzly available (15, 15, and 20%) versus rounds where there was no grizzly to find (40, 80, and 80%). Maverick showed a tendency to false respond on familiar black bear (71%) more often than unfamiliar black bear (29%). Zach and Kelly false responded more often on new black bear (64 and 67%) than on known black bear (36 and 33%).

 

Training and testing also resulted in a qualitative assessment of characteristics of detection dog candidates. Of the nine dogs that began training, three were tested (Maverick, Kelly, and Zach). Three dogs were progressing in training but had not yet reached a testable level at the time of this writing. Three dogs had been dropped from training. Of the dropped dogs, one housepet had become bored with the repetitive nature of the scent box and began pawing at each hole and lying down or going to the door. His aptitude for learning was great; he had learned the odor quickly and maintained high ball drive throughout. The second dog, also a housepet, displayed great ball drive while at home but was unable to maintain drive when at the training facility. The third dog was a narcotic detection dog candidate who maintained ball drive, achieved odor recognition easily and remained interested in the scent box. While her aptitude was sufficient, her attitude hampered her success. She displayed an unwillingness to work with the handler consistently in a methodical progression along the scent box.

 

Differing characteristics were also apparent among the dogs who completed training. Maverick had previously completed 5 weeks of narcotic training but did not finish due to injury. Kelly had been through several weeks of narcotic training but was partnered with handlers who did not build her working confidence. Though Maverick achieved 65% hit rate in our testing and Kelly 85%, Maverick was the more confident worker and much easier to read. Kelly required a higher level of handling skills because she was prone to guess or give unreadable responses. Given equal amounts of training we expect that Maverick would have achieved or surpassed Kelly’s success rate based on their working styles. Zach had no experience with scent work, but received the most training for this testing. His personality aided his progress in that it was in his nature to please people and he developed rituals easily and enjoyed sticking to a routine. Thus he remained eager to approach the scent box, and worked methodically.

 

Discussion

This experiment showed that trained dogs can distinguish grizzly bear from black bear scat. From a professional training standpoint, a dog with a 70% hit rate successfully completed training. A 90% hit rate is required for Washington state certification. Kelly and Zach were above 70% (85%) and nearly reached certification level. Maverick nearly reached 70% (65%). This success rate is notable because of two primary factors: short training time and lack of adequate exposure to a large number of diverse scats. In the narcotic detection program taught by B.D, dogs are trained for 6 weeks (240 hours) to locate and alert to 4 major odors and their variations.  Each dog completes approximately 600 repetitions using approximately 100 different training aids (target scents) in which amount and packaging is varied. Maverick and Kelly were trained 1 and 4 days respectively, or approximately 1-2 hours and 4-6 hours. However, both of them have completed approximately 5 weeks of narcotics training. Thus they did not have to learn how to use the scent box, but rather concentrated on learning the odor. Zach, who had no previous scent work, had 11 days of training, or approximately 15 hours. B.D predicts that it would take 2-3 days of intense training followed by 4-6 weeks of 3-times-weekly training to ready a dog for the professional application of this type of odor discrimination. Short training sessions would be imperative due to the repetitive nature of this training, in which the scent box is always the item a dog searches.

 

In addition to a much longer training period, work-ready dogs would need a great deal more scat diversity than was offered to the dogs in this trial. Clearly, scat from two grizzlies was not enough diversity in odor to allow the dog to generalize the scent as these dogs showed a greater accuracy in detecting the scat they had been trained on than new scat. Trained narcotic detection dogs—who  undergo a great deal of training and are exposed to a much larger diversity of target odors—experience increased detection success rates once they have ample professional working experience.   Detection success is proportional to training time and diversity of scent exposure. This is an expected and accepted relationship among detection canine professionals, and is of the utmost importance to this study as well.

 

Of the new scats encountered during testing, one was particularly troublesome for all three dogs. Kelly missed it the first time it appeared, she then hit it the next two times it appeared, indicating that she learned that scat in just one encounter with it. Maverick and Zach both missed that scat all three times that it appeared. In the case of Zach, this scat was the only grizzly he missed. This scat was from a captive individual who had been fed vegetation. The individuals used during training ate primarily chow with some vegetation added. The second new individual was hit with a higher success rate (all 5 times it appeared Zach and Kelly hit it, 3 of the 5 Maverick hit it). This second individual was a cub that was fed exclusively chow. The lack of diversity of scat and not having any wild scat in our study doesn’t allow us to make broad inferences about the importance of diet in the dogs’ ability to learn grizzly scat odor. However, this observation indicates that diet does matter to some degree. In the face of limited exposure it may be easier for the dog to detect a grizzly with a similar diet to the diet expressed in training samples. We expect that increased exposure to scat diversity would reduce diet as a selection factor.

 

The rounds which contained no grizzly bear scat were problematic for all dogs. Fifty-five percent of all false responses happened during these “blank” rounds. This is especially impressive when taken into account that blank rounds accounted for only 20% of all rounds. It is worth noting that we did not consider false responses to be as serious of an error as missing grizzly scat. Presumably, professional distinction dogs would pre-screen scat before being sent to the lab. The inclusion of a few non-target scats would not significantly burden many studies.

 

Maverick was the only dog who had 3 blank rounds which were correct, he falsely responded on his other two blank rounds. Kelly and Zach gave at least one false response on each of the five blank rounds. Kelly and Zach were also similar in that when they falsely responded they were more likely to chose a black bear scat that was new to them, indicating that when unsure they selected something entirely unfamiliar. This is in contrast to Maverick, who when unsure selected black bear that was familiar to him from training. The disproportionate amount of false responses on blank rounds made us believe that the problem was one of training. Blank rounds made the dog work yet didn’t result in a reward, an inherently disappointing outcome for the dog. Because of this, we did not introduce the concept of blank rounds until the dogs were about ¾ of the way through their training, setting up the precedent that there was always grizzly to be found. Additionally, in the narcotic training that Maverick and Kelly had previously, there was always target scent in the scent box. In future studies it is imperative that dogs be introduced to the concept of blank rounds in the initial stages of training. 

 

Conclusion

With an overall successful hit rate of 65% for Maverick and 85% for Kelly and Zach, it is clear that dogs have the ability to select grizzly bear scat out of a line up with black bear scat. A comprehensive training schedule of 4-6 weeks, which includes several dozen different (and wild) grizzly bear scats and a proportional amount of black bear scat is recommended for a dog who is to be used professionally in this type of detection work. It is expected that this diversity in scat exposure would increase the dog’s ability to generalize grizzly scat odor, thereby increasing the dog’s ability to hit on unfamiliar scat and reduce any preferences for certain diet.  In real detection work there would likely be many rounds in which no grizzly scat was in the scent box. Therefore, it would be imperative to begin training for blank rounds very early in the training progression. Compared to the variety of work situations encountered by other professional detection dogs, these dogs would work repeatedly at one location—the scent box. Dogs selected for this training would need to possess traits that allowed them to succeed with this type of repetition. Such characteristics include high ball drive, methodical working style, moderate energy level, and a willingness to work consistently with the handler. Given the success of this initial study, we feel confident that these training suggestions can produce competent scat discrimination dogs to assist researchers and managers in their worthwhile goals.

 

Acknowledgements

This work was funded in part by an IBS-CORE Undergraduate Research Fellowship to Aimee Hurt through a grant from the Howard Hughes Medical Institute to the University of Montana. Additional funding was granted by the Matthew Hansen Foundation. A.H. would like to thank all of the generous dog owners who entrusted their beloved dogs to her care: Scott and Colleen, Kathryn and Jason, Carol B., Carol S., George, Rosemary, and Mariah. Thanks to the busy professionals who took the extra time to gather scat for this training: Rick Mace, Charlie Robbins, and Ed Cleveland. Thanks to IBS-CORE staff, Kris Nord, and Brian Steele. Thanks to Meg, Tyler, Karrin, and Deb for emotional and logistical support. A.H. would like to offer special thanks to E.G. for vouching for this endeavor and B.D. who was wangled into this project without even knowing it.

 

Literature Cited

Brisbin, I.L. and Austad, S.N. 1991. Testing the individual odour theory of canine         olfaction. Animal Behaviour 42(1):63-69.

 

Hunt, C.L. 1997. Proposal for the “Partners in Life” program. Wind River Bear Institute.           Heber City, UT.

 

Smith, D.A. 2000. (kit fox research) in press.

 

Sturdvian, C. 1993. Locating wolves with a dog trained in tracking and scent-   discrimination. (unpublished manuscript).

 

Syrotuck, W. 1972.Scent and the scenting dog. American Publications, Rome, NY.

 

Wasser, S.K. and Davenport, B. 1998. (black bear research) unpublished.


 

Table 1. Scats used in discrimination tests

 

Training

Known individual, new scat

New individual

TOTAL

Grizzly captive

6

5

2

13

Black captive

5

3

0

8

Black wild

5

0

7

12

TOTAL

16

8

9

33

 

 

Table 2. Hit and False Response Rate

Dog

Hit rate

False response rate

 

Known

(out of 9)

Unknown

(out of 2)

New on testing day (out of 6)

 

New from known bears (out of 3)

Total hit

(out of 20)

FR when griz available

(out of 20)**

FR when no griz available

(out of 5)**

FR on new black bear

FR on known black bear

Maverick

 

9 (100%)

0 (0%)

3 (50%)

1 (33%)

13 (65%)

3 (15%)

2 (40%)

2 of 7 (29%)

5 of 7 (71%)

Zach

 

9 (100%)

1 (50%)

4 (67%)

3 (100%)

17 (85%)

3 (15%)

4 (80%)

7 of 11 (64%)

4 of 11 (36%)

Kelly

8 (89%)

1 (50%)

6 (100%)

2 (67%)

17 (85%)

4 (20%)

4 (80%)

6 of 9 (67%)

3 of 9 (33%)

** counted per round, thus multiple false responses in any one round counted only once

 

 

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